Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

Two-photon Fluorescence Endomicroscopy

Ultra-large field-of-view two-photon microscopy.

We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of t...

Sensitivity Enhancement of Fiber Optic Diesel Adulteration Detection Sensor Using Stripped Clad SBend Section

A novel geometry for enhancing the sensitivity of intensity modulated refractometric fiber optic sensor for detection of adulteration level in diesel by kerosene is proposed. In this multimode plastic optical fiber is uncladded for specific length and bent into S shape. This geometry is simulated and analyzed using Beam Propagation Method in Beam prop RSOFT software. When sensor is immersed in ...

Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo.

We present a small, lightweight two-photon fiberscope and demonstrate its suitability for functional imaging in the intact brain. Our device consists of a hollow-core photonic crystal fiber for efficient delivery of near-IR femtosecond laser pulses, a spiral fiber-scanner for resonant beam steering, and a gradient-index lens system for fluorescence excitation, dichroic beam splitting, and signa...

Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy

In this paper, we present a strategy to improve delivery of femtosecond laser pulses from a regenerative amplifier through a hollow core photonic crystal fiber for temporally focused wide-field two-photon endomicroscopy. For endomicroscope application, wide-field two-photon excitation has the advantage of requiring no scanning in the distal end. However, wide-field two-photon excitation require...